Brad Fletcher, M.D.

Brad S Fletcher, M.D., Ph.D.

(352) 265-8199

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Publications

A case study of 10 patients administered HBOC-201 in high doses over a prolonged period: outcomes during severe anemia when transfusion is not an option
Transfusion
2020

Atypical haemolytic uremic syndrome secondary to acute pancreatitis: a unique presentation

2019

Tem8/Antxr1-Specific Car T Cells As a Targeted Therapy for Triple-Negative Breast Cancer
Cancer Research
2018

Idiopathic Pulmonary Vein Thrombosis – A presentation of two case reports and review of the current literature

2017

Clinical Validation of R-T Estimation for Coaguchek Xs Inr Results
Annals of Pharmacotherapy
2016

Two Is Better Than One: Adoptive T Cell Therapy Targeting Tumor Antigens and the Tumor Endothelium Potentiates T Cell Activation and Cytolytic Activity
Cancer Research
2014

Tumor Endothelial Marker 8 Expression in Triple-Negative Breast Cancer
Anticancer Research
2011

The Promise of Nanotechnology for Solving Clinical Problems in Breast Cancer
Journal of Surgical Oncology
2011

Advancement in Non-Viral Gene Therapy for Hemophilia
Journal of Genetic Syndromes and Gene Therapy
2011

Targeting Tumor Endothelial Marker 8 in the Tumor Vasculature of Colorectal Carcinomas in Mice
Cancer Research
2009

Specific Disruption of Tumor Vessels By Targeting Tem8
Molecular Therapy
2009

Piggybac Transposon-Based Inducible Gene Expression in Vivo After Somatic Cell Gene Transfer
Molecular Therapy
2009

Molecular Evolution of a Novel Hyperactive Sleeping Beauty Transposase Enables Robust Stable Gene Transfer in Vertebrates
Nature Genetics
2009

Indoleamine 2,3-Dioxygenase Attenuates Inhibitor Development in Gene-Therapy-Treated Hemophilia a Mice
Gene Therapy
2009

Bcl-2 Mediated Modulation of Vascularization in Prostate Cancer Xenografts
Prostate
2009

Development and Validation of An Approach To Produce Large-Scale Quantities of Cpg-Methylated Plasmid Dna
Microbial Biotechnology
2008

Sustained Fviii Expression And Phenotypic Correction Of Hemophilia A In Neonatal Mice Using An Endothelial-Targeted Sleeping Beauty Transposon.
Molecular Therapy : The Journal Of The American Society Of Gene Therapy
2006

Sleeping Beauty-Mediated Enos Gene Therapy Attenuates Monocrotaline-Induced Pulmonary Hypertension In Rats.
The FASEB's Journal
2006

Sleeping Beauty-Based Gene Therapy With Indoleamine 2,3-Dioxygenase Inhibits Lung Allograft Fibrosis.
The FASEB's Journal
2006

Sleeping Beauty Transposon-Mediated Nonviral Gene Therapy.
BioDrugs: Clinical Immunotherapeutics, Biopharmaceuticals, and Gene Therapy
2006

Novel Action of Indoleamine 2,3-Dioxygenase Attenuating Acute Lung Allograft Injury
Am J Respir Crit Care Med
2006

The Wd-Repeat Protein Grwd1: Potential Roles In Myeloid Differentiation And Ribosome Biogenesis.
Genomics
2005

Molecular alterations resulting from frameshift mutations in peripheral myelin protein 22: Implications for neuropathy severity.
J Neurosci Res
2005

Modulation Of Epithelial Morphology, Monolayer Permeability, And Cell Migration By Growth Arrest Specific 3/Peripheral Myelin Protein 22.
Molecular Biology of the Cell
2005

Impaired Proteasome Activity And Accumulation Of Ubiquitinated Substrates In A Hereditary Neuropathy Model.
Journal of Neurochemistry
2005

Hyperactive transposase mutants of the Sleeping Beauty transposon.
Molecular Therapy
2005

Transposon-based RNAi delivery system for generating knockdown cell lines
Biochemical and Biophysical Research Communications
2004

Potent anti-tumor effects of an active site mutant of human MnSOD: Evolutionary conservation of product inhibition
Journal of Biological Chemistry
2004

Glucose Deprivation Enhances Targeting Of Glut1 To Lipid Rafts In 3T3-L1 Adipocytes.
American journal of physiology. Endocrinology and metabolism
2004

Endothelial Targeting Of The Sleeping Beauty Transposon Within Lung.
Molecular Therapy : The Journal Of The American Society Of Gene Therapy
2004

Construction of a transposon-based short hairpin RNA delivery system for the generation of stable gene specific knockdown cell lines
Biochemical and Biophysical Research Communications
2004

Glycogen phosphorylase does not regulate glucose transport activity in 3T3-L1 adipocytes
Biochimica Et Biophysica Acta-Molecular Basis of Disease
2002

Functional Cloning Of Spin-2, A Nuclear Anti-Apoptotic Protein With Roles In Cell Cycle Progression.
Leukemia
2002

Peripheral myelin protein 22 is a constituent of intercellular junctions in epithelia.
Proceedings of the National Academy of Sciences
2001

Asparagine Synthetase Expression Alone Is Sufficient To Induce L-Asparaginase Resistance In Molt-4 Human Leukaemia Cells.
The Biochemical journal
2001

Transcriptional Induction Of Prostaglandin G/H Synthase-2 By Basic Fibroblast Growth Factor.
The Journal Of Clinical Investigation
1995

V-Src Induction Of The Tis10/Pgs2 Prostaglandin Synthase Gene Is Mediated By An Atf/Cre Transcription Response Element.
Molecular And Cellular Biology
1994

The Tis Genes, Primary Response Genes Induced By Growth Factors And Tumor Promoters In 3T3 Cells.

1994

Platelet-Activating Factor And Retinoic Acid Synergistically Activate The Inducible Prostaglandin Synthase Gene.
Proceedings of the National Academy of Sciences
1994

Tis10, A Mitogen-Inducible Glucocorticoid-Inhibited Gene That Encodes A Second Prostaglandin Synthase/Cyclooxygenase Enzyme.
Journal of Lipid Mediators and Cell Signalling
1993

Expression Of The Protein Product Of The Prostaglandin Synthase-2/Tis10 Gene In Mitogen-Stimulated Swiss 3T3 Cells.
Journal of Biological Chemistry
1993

Chromosomal Organization Of The Inducible And Constitutive Prostaglandin Synthase/Cyclooxygenase Genes In Mouse.
Genomics
1993

Structure Of The Mitogen-Inducible Tis10 Gene And Demonstration That The Tis10-Encoded Protein Is A Functional Prostaglandin G/H Synthase.
Journal of Biological Chemistry
1992

Tis10, A Phorbol Ester Tumor Promoter-Inducible Mrna From Swiss 3T3 Cells, Encodes A Novel Prostaglandin Synthase/Cyclooxygenase Homologue.
Journal of Biological Chemistry
1991

The Tis11 Primary Response Gene Is A Member Of A Gene Family That Encodes Proteins With A Highly Conserved Sequence Containing An Unusual Cys-His Repeat.
Molecular And Cellular Biology
1991

Structure And Expression Of Tis21, A Primary Response Gene Induced By Growth Factors And Tumor Promoters.
Journal of Biological Chemistry
1991

Research Interests

Dr. Fletcher’s major effort is focused on biomedical research using transposons as vehicles to facilitate long-term gene expression following non-viral gene delivery. This approach has been used to treat various conditions in animal models including hemophilia, pulmonary hypertension and acute graft rejection. Recent work focuses on targeting tumor vasculature in solid tumors.

Dr. Fletcher’s clinical activities include attending the inpatient hematology consultation service at Shands Hospital and outpatient clinical hematology and follow-up at both the Shands Hospital and the Malcom Randall Veterans Administration Medical Center.