Michael Bubb, M.D.

Michael R Bubb, M.D.

(352) 265-4846

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Publications

The Mismatch of Nutrition and Lifestyle Beliefs and Actions Among Physicians: A Wake-Up Call.
American journal of lifestyle medicine
2020

Do Hydroxychloroquine Blood Levels and Dose Identify Different Populations at Risk of Retinopathy? Comment on the Article by Petri et al.
Arthritis & rheumatology (Hoboken, N.J.)
2020

Excess Deaths Upon Cessation of Xanthine Oxidase Inhibitor Treatment-Data From the Cardiovascular Safety of Febuxostat and Allopurinol in Patients With Gout and Cardiovascular Morbidities Trial: Comment on the Article by Choi et al.
Arthritis & rheumatology (Hoboken, N.J.)
2019

Efficacy of Warfarin Anticoagulation and Incident Dementia: Does Cognitive Impairment Reduce Efficacy?
Mayo Clinic proceedings
2018

Does Digital Rectal Examination Reduce Subsequent Hospitalizations and Endoscopies or Vice Versa?
The American journal of medicine
2018

Sjögren's syndrome-associated microRNAs in CD14(+) monocytes unveils targeted TGFβ signaling.
Arthritis research & therapy
2016

Muscarinic type 3 receptor autoantibodies are associated with anti-SSA/Ro autoantibodies in Sjögren's syndrome.
Journal of immunological methods
2016

In Reply I-Risk of Disseminated Disease in Immunosuppressed Patients Receiving Live Zoster Vaccine.
Mayo Clinic proceedings
2016

Risk of Disseminated Varicella Zoster in Immunosuppressed Patients Receiving Zoster Vaccination.
Mayo Clinic proceedings
2015

Biochemical and biophysical properties of a putative hub protein expressed by vaccinia virus.
The Journal of biological chemistry
2013

Anti-Th/To Antibodies in Various Systemic Rheumatic Diseases Screened By Anti-Rpp25 Elisa.
Arthritis and Rheumatism
2013

Potential, but unobserved, adverse cardiovascular effects from endurance exercise.
Mayo Clinic proceedings
2012

Clinical Features Associated With Anti-Th/To in Non-Scleroderma Patients - Sine Scleroderma?
Arthritis and Rheumatism
2012

Gender and ethnicity differences in the prevalence of scleroderma-related autoantibodies.
Clinical rheumatology
2011

Frequent coexistence of anti-topoisomerase I and anti-U1RNP autoantibodies in African American patients associated with mild skin involvement: a retrospective clinical study.
Arthritis research & therapy
2011

Atypical clinical presentation of a subset of patients with anti-RNA polymerase III--non-scleroderma cases associated with dominant RNA polymerase I reactivity and nucleolar staining.
Arthritis research & therapy
2011

Identification of a cofilin-like actin-binding site on translationally controlled tumor protein (TCTP).
FEBS letters
2010

Changes in the Visiting Medical Student Clerkship Program at Mayo Clinic.
Mayo Clinic proceedings
2010

How depolymerization can promote polymerization: the case of actin and profilin.
BioEssays : news and reviews in molecular, cellular and developmental biology
2009

Genetically elevated C-reactive protein and vascular disease.
The New England journal of medicine
2009

Exogenous thymosin beta4 prevents apoptosis in human intervertebral annulus cells in vitro.
Biotechnic & histochemistry : official publication of the Biological Stain Commission
2009

Air pollution and life expectancy.
The New England journal of medicine
2009

Upregulated miR-146a expression in peripheral blood mononuclear cells from rheumatoid arthritis patients.
Arthritis research & therapy
2008

Significant Racial and Gender Differences in Specificity of Scleroderma-Related Autoantibodies
Arthritis and Rheumatism
2008

Effect of profilin on actin critical concentration: a theoretical analysis.
Biophysical journal
2008

Clinical Significance of Autoantibodies To Topoisomerase I and Rna Polymerase Iii in An Unselected Rheumatic Disease Population
Arthritis and Rheumatism
2008

Thymosin beta4: actin regulation and more.
Annals of the New York Academy of Sciences
2007

One-trial in vitro conditioning regulates an association between the beta-thymosin repeat protein Csp24 and actin.
Neuroscience
2007

Identification of single and double sites of phosphorylation by ECD FT-ICR/MS in peptides related to the phosphorylation site domain of the myristoylated alanine-rich C kinase protein.
Journal of the American Society for Mass Spectrometry
2007

Tropomodulin 3 binds to actin monomers.
The Journal of biological chemistry
2006

Profilin: emerging concepts and lingering misconceptions.
Trends in biochemical sciences
2006

Nucleolar staining cannot be used as a screening test for the scleroderma marker anti-RNA polymerase I/III antibodies.
Arthritis and rheumatism
2006

A CapG gain-of-function mutant reveals critical structural and functional determinants for actin filament severing.
The EMBO journal
2006

MARCKS is a natively unfolded protein with an inaccessible actin-binding site: evidence for long-range intramolecular interactions.
The Journal of biological chemistry
2005

Interactions between vacuolar H+-ATPases and microfilaments in osteoclasts.
Journal of bioenergetics and biomembranes
2005

Vacuolar H+-ATPase binding to microfilaments: regulation in response to phosphatidylinositol 3-kinase activity and detailed characterization of the actin-binding site in subunit B.
The Journal of biological chemistry
2004

Effects of profilin and thymosin beta4 on the critical concentration of actin demonstrated in vitro and in cell extracts with a novel direct assay.
The Journal of biological chemistry
2004

Actin crystal dynamics: structural implications for F-actin nucleation, polymerization, and branching mediated by the anti-parallel dimer.
Journal of structural biology
2004

Thymosin beta 4 interactions.
Vitamins and hormones
2003

Regulation of endothelial nitric oxide synthase by the actin cytoskeleton.
American journal of physiology. Cell physiology
2003

Depolymerization of actin filaments by profilin. Effects of profilin on capping protein function.
The Journal of biological chemistry
2003

Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolution.
The Journal of biological chemistry
2002

Control of actin dynamics by proteins made of beta-thymosin repeats: the actobindin family.
The Journal of biological chemistry
2002

Benzo(a)pyrene, but not 2,3,7,8-tetrachlorodibenzo-p-dioxin, alters cell adhesion proteins in human uterine RL95-2 cells.
Biochemical and biophysical research communications
2002

Formation and implications of a ternary complex of profilin, thymosin beta 4, and actin.
The Journal of biological chemistry
2001

Cytoskeletal regulation of the L-arginine/NO pathway in pulmonary artery endothelial cells.
American journal of physiology. Lung cellular and molecular physiology
2001

Actin filament cross-linking by MARCKS: characterization of two actin-binding sites within the phosphorylation site domain.
The Journal of biological chemistry
2001

Effects of jasplakinolide on the kinetics of actin polymerization. An explanation for certain in vivo observations.
The Journal of biological chemistry
2000

Actin-latrunculin A structure and function. Differential modulation of actin-binding protein function by latrunculin A.
The Journal of biological chemistry
2000

Replacement of the aortic root in Marfan's syndrome.
The New England journal of medicine
1999

Phosphorylation-dependent conformational changes induce a switch in the actin-binding function of MARCKS.
The Journal of biological chemistry
1999

New anti-actin drugs in the study of the organization and function of the actin cytoskeleton.
Microscopy research and technique
1999

Lengthening the second stalk of F(1)F(0) ATP synthase in Escherichia coli.
The Journal of biological chemistry
1999

Inclusion body myositis long after dermatomyositis: a report of two cases.
Clinical and experimental rheumatology
1999

Active compression-decompression cardiopulmonary resuscitation.
The New England journal of medicine
1999

Use of the F-actin-binding drugs, misakinolide A and swinholide A.
Methods in enzymology
1998

Localization of actobindin, profilin I, profilin II, and phosphatidylinositol-4,5-bisphosphate (PIP2) in Acanthamoeba castellanii.
Cell motility and the cytoskeleton
1998

Formation of the b subunit dimer is necessary for interaction with F1-ATPase.
Biochemistry
1998

Profilin interacts with the Gly-Pro-Pro-Pro-Pro-Pro sequences of vasodilator-stimulated phosphoprotein (VASP): implications for actin-based Listeria motility.
Biochemistry
1997

Misakinolide A is a marine macrolide that caps but does not sever filamentous actin.
The Journal of biological chemistry
1997

Gelsolin activates DNase I in vitro and cystic fibrosis sputum.
Biochemistry
1997

Self-assembly of the brain MAP-2 microtubule-binding region into polymeric structures resembling Alzheimer filaments.
Biochemical and biophysical research communications
1996

In vitro polymerization of embryonic MAP-2c and fragments of the MAP-2 microtubule binding region into structures resembling paired helical filaments.
The Journal of biological chemistry
1996

Assembly of MAP-2c and MTBR Fragments into Polymers Resembling Paired Helical Filaments
Journal of Biological Chemistry
1996

Swinholide A is a microfilament disrupting marine toxin that stabilizes actin dimers and severs actin filaments.
The Journal of biological chemistry
1995

Kinetic model for the inhibition of actin polymerization by actobindin.
Biochemistry
1995

Jasplakinolide, a cytotoxic natural product, induces actin polymerization and competitively inhibits the binding of phalloidin to F-actin.
The Journal of biological chemistry
1994

Actobindin induces the accumulation of actin dimers that neither nucleate polymerization nor self-associate.
The Journal of biological chemistry
1994

Actobindin binds with high affinity to a covalently cross-linked actin dimer.
The Journal of biological chemistry
1994

Rabbit skeletal muscle actin behaves differently than Acanthamoeba actin when added to soluble extracts of Acanthamoeba castellanii.
Biochemical and biophysical research communications
1993

Profilin-actin complexes directly elongate actin filaments at the barbed end.
Biochemistry
1992

Limited proteolysis reveals a structural difference in the globular head domains of dephosphorylated and phosphorylated Acanthamoeba myosin II.
The Journal of biological chemistry
1992

The interfaces of actin and Acanthamoeba actobindin. Identification of a new actin-binding motif.
The Journal of biological chemistry
1991

The interaction of monomeric actin with two binding sites on Acanthamoeba actobindin.
The Journal of biological chemistry
1991

Purification of actobindin from Acanthamoeba castellanii.
Methods in enzymology
1991

The covalent structure of Acanthamoeba actobindin.
The Journal of biological chemistry
1990

The isolation of immunogenically pure IgM from Cohn fraction 3 of pooled normal human plasma.
Immunochemistry
1973

Research Interests

RESEARCH INTERESTS

Dr. Bubb's laboratory studies the regulation of actin polymerization in cell motility. We investigate the cell biology and biochemistry of actin-binding proteins specifically as they pertain to such topics as apoptosis, angiogenesis, immunology and cancer biology. We also study pharmacologic agents that affect actin polymerization. We use a variety of biophysical techniques to answer specific questions regarding mechanisms controlling cell motility, including time-dependent fluorescence spectroscopy and analytical ultracentrifugation.

Specific ongoing projects include (1) a study of the MARCKS protein as it relates to neurodevelopment, synaptic plasticity and memory, (2) a study of the structure and immunomodulatory properties of thymosin 4, and (3) the development of cancer chemotherapeutic agents that target the actin cytoskeleton, and (4) a study of predicted outcomes and response to therapy in rheumatoid arthritis based on the role of TNF-alpha in cytoskeletal dynamics.

ABAKUS or Actin Binding Activities and Kinetic solUtionS, an interactive model of actin polymerization developed by our laboratory.

ACTIVE RESEARCH STUDIES

A randomized, double-blind, placebo-controlled multicenter study of secukinumab to evaluate the safety, tolerability and efficacy up to 2 years in patients with active nonradiographic axial spondyloarthritis.

  • The purpose of this study is to determine whether the use of the study drug, secukinumab, can help treat patients who have non-radiographic ankylosing spondylitis. This study is currently actively enrolling. Further information is available at www.clinicaltrials.gov.
  • Physicians and patients are welcome to contact us for more information.
  • The principal investigator, Dr. Michael Bubb, can be reached at the rheumatology office, 352.294.8202.

Active Research Studies