Protein protects lung cancer cells from efforts to fix or kill them
A protein that helps lung cancer cells thrive appears to do so by blocking healthy cells' ability to fix themselves when radiation or chemicals such as nicotine damage their DNA, according to a University of Florida study to be published Friday (Feb. 29) in the journal Molecular Cell.
High levels of the protein, known as Bc12, are found in the cells of lung cancer patients who smoke.
Previous UF research has shown that nicotine activates the protein, which helps tumor cells live long past their natural lifespan and resist chemotherapy. The new findings explain how the protein enables cancer cells to circumvent the body's own efforts to change them back into healthy cells - or evade treatments designed to kill them.
Cancer is frequently associated with the accumulation of genetic aberrations in cells' chromosomes. If these damaged cells can't access their built-in repair system and subsequently survive long enough to divide and multiply, they pass along their mutations.
"If a cell experiences DNA damage, often that DNA can be repaired. But we found that Bc12 can block the DNA repair mechanism, which promotes tumor formation and genetic instability," said Xingming Deng, M.D., Ph.D., an assistant professor in UF's College of Medicine who is affiliated with the UF Shands Cancer Center. "This is a very important fundamental mechanism that explains why this protein has (a cancer-forming) function."
Researchers say just one cell that develops a genetic mutation and is unable to repair itself could be enough for a full-blown tumor to develop.
"Lung cancer is the No. 1 killer of all cancer types; it is the most dangerous," Deng said. "We wanted to find a way to treat lung cancer, how to prevent lung cancer, because lung cancer prognosis is very poor."
Nearly 162,000 people will die from lung cancer in 2008, accounting for about 29 percent of all cancer deaths, according to the American Cancer Society. More people die of lung cancer than of colon, breast and prostate cancers combined.
In the study, UF scientists performed a series of laboratory experiments on lung cancer cells in culture that illuminated the molecular chain of events that allows Bc12 to disrupt DNA repair.
Deng also plans to explore the possibility that nicotine-induced activation of Bc12 can be blocked to increase chemotherapy's effectiveness.
"This will probably help us in the future find ways to prevent tumors," said Deng, adding that the protein could be a target for drug development. "We can target this mechanism and somehow find a way to prevent tumor formation."
The research was funded by about $1.2 million in grants from the National Institutes of Health and the Flight Attendant Medical Research Institute. FAMRI was established in 1997 as a result of a $300 million settlement between airline flight attendants and the tobacco industry. The nonprofit organization awards grants for research focusing on smoking-related illnesses.